EMPIAR-11043 - Architecture of the human erythrocyte ankyrin-1 complex [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-11043

Architecture of the human erythrocyte ankyrin-1 complex [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Architecture of the human erythrocyte ankyrin-1 complex

Vallese F, Kim K, Yen LY, Johnston JD, Noble AJ, Calì T, Clarke OB

Nature structural & molecular biology 29 (2022) 706-718

PMID: 35835865

DOI: 10.1038/s41594-022-00792-w

Related EMDB entry:

EMD-26943

Deposited:

2022-02-08

Released:

2022-08-08

Last modified:

2022-09-09

Imageset size:

5.24 TB

Imageset DOI:

10.6019/EMPIAR-11043

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - unprocessed

Image Sets

1. Unaligned multi-frame movies of ankyrin-1 complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14926

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

The micrographs are of 50 raw movie frames accumulating 58.2 electrons per squared Angstrom in a 2.5 second exposure.
Particle positions are in .star files with X and Y coordinates in 3rd and 4th column respectively.

consensus_particles_stack.mrcs contains the particle images of the ankyrin-1 complex consensus particle set.

Band3_GPA_particles.star contains the particle coordinates and CTF parameters of the band3-GPA partcle set.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Aligned micrographs of ankyrin-1 complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

14926

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

The raw movie frames are aligned with patch motion correction in cryoSPARC. (50 frames, total dose: 58.2e-/A2, Cs=2.7)
Particle positions are in .star files with X and Y coordinates in 3rd and 4th column respectively.
consensus_particles_stack.mrcs contains the particle images of the ankyrin-1 complex consensus particle set.
Band3_GPA_particles.star contains the particle coordinates and CTF parameters of the band3-GPA partcle set.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Consensus particle stack for the ankyrin-1 complex

Category:

picked particles - single frame - unprocessed

Image format:

MRCS

No. of images or tilt series:

710437

Image size:

(450, 450)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.83, 0.83)

Details:

710437 particle image stack is saved as consensus_particles_stack.mrcs

Particle coordinates and CTF parameters are saved in consensus_particles_stack.star

Pixel size: 0.83 Å/pix
Box size: 450 pix

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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