EMPIAR-11010 - Cryo-electron microscopy reconstruction of PCAT1 in three distinct inward-facing conformations during ATP turnover condition [11966 multi-frame micrographs composed of 50 frames each in TIFF format]

EMPIAR-11010

Cryo-electron microscopy reconstruction of PCAT1 in three distinct inward-facing conformations during ATP turnover condition [11966 multi-frame micrographs composed of 50 frames each in TIFF format]

Publication:

Structures of the peptidase-containing ABC transporter PCAT1 under equilibrium and nonequilibrium conditions

Kieuvongngam V, Chen J

Proceedings of the National Academy of Sciences of the United States of America 119 (2022)

PMID: 35074919

DOI: 10.1073/pnas.2120534119

Related EMDB entry:

EMD-25696 EMD-25695 EMD-25697

Deposited:

2021-11-08

Released:

2022-06-21

Last modified:

2022-06-21

Imageset size:

5.75 TB

Imageset DOI:

10.6019/EMPIAR-11010

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned, uncorrected movies of PCAT1 in ATP active turnover condition

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11966

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.515, 0.515)

Details:

There are two datasets separated in subdirectories. Each set contains super-resolution, raw, uncorrected movies and gain references that have been flipped and rotated by 90 degrees for use. The super-resolution pixel size is 0.515 before binning.

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Citations

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Kim K, Li H, Clarke OB. (2025)

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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