EMPIAR-10981 - CryoET of E. coli prepared with the Waffle Method [1504 multi-frame micrographs composed of 14 frames each in TIFF format]

EMPIAR-10981

CryoET of E. coli prepared with the Waffle Method [1504 multi-frame micrographs composed of 14 frames each in TIFF format]

Publication:

Waffle Method: A general and flexible approach for improving throughput in FIB-milling

Kelley K, Jaroenlak P, Raczkowski, AM, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ

bioRxiv (2020)

DOI: 10.1101/2020.10.28.359372

Related EMDB entry:

EMD-26396

Deposited:

2022-03-09

Released:

2022-03-21

Last modified:

2022-03-21

Imageset size:

94.00 GB

Imageset DOI:

10.6019/EMPIAR-10981

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs for each tilt image

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1504

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(2.077, 2.077)

Details:

Unaligned K3 tif tilt image frame stacks. Frame number increases from 14 at 0 degrees to 23 at high tilts to account for increasing thickness; dose increases from 4.87 to 8.00 e/A^2. Collected bi-directionally from [0:53.2] then [0:-53.2] with 2.8 degree increments.
Gain references are in the references/ folder.

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Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

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