EMPIAR-10981
CryoET of E. coli prepared with the Waffle Method [1504 multi-frame micrographs composed of 14 frames each in TIFF format]
Publication:

Waffle Method: A general and flexible approach for improving throughput in FIB-milling

Kelley K, Jaroenlak P, Raczkowski, AM, Eng ET, Bhabha G, Potter CS, Carragher B, Noble AJ

bioRxiv (2020)

Related EMDB entry:
Deposited:
2022-03-09
Released:
2022-03-21
Last modified:
2022-03-21
Imageset size:
94.00 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs for each tilt image
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1504
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(2.077, 2.077)
Details:
Unaligned K3 tif tilt image frame stacks. Frame number increases from 14 at 0 degrees to 23 at high tilts to account for increasing thickness; dose increases from 4.87 to 8.00 e/A^2. Collected bi-directionally from [0:53.2] then [0:-53.2] with 2.8 degree increments.
Gain references are in the references/ folder.
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