EMPIAR-10818 - Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate [3879 multi-frame micrographs composed of 50 frames each in MRC format]

EMPIAR-10818

Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate [3879 multi-frame micrographs composed of 50 frames each in MRC format]

Publication:

Structural basis of substrate recognition by a polypeptide processing and secretion transporter

Kieuvongngam V, Olinares PDB, Palillo A, Oldham ML, Chait BT, Chen J

eLife 9 (2020)

PMID: 31934861

DOI: 10.7554/elife.51492

Related EMDB entry:

EMD-21132

Deposited:

2021-09-10

Released:

2021-11-26

Last modified:

2021-11-26

Imageset size:

2.00 TB

Imageset DOI:

10.6019/EMPIAR-10818

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned and uncorrected multiframe movies of PCAT1 bound to its CtA peptide substrate

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3879

Image size:

(7420, 7676)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(0.545, 0.545)

Details:

The micrographs are the raw 50-frame tif movies collected with a super-resolution pixel size 0.545 Angstrom (1.09 Angstrom after binning). The dataset is separated into two subdirectories. The mrc movies has been compressed to bz2 format. The gain reference files are in a separate directory as the movie datasets

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Citations

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Kim K, Li H, Clarke OB. (2025)

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Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

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Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

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