EMPIAR-10818
Cryo-electron microscopy reconstruction of PCAT1 bound to its CtA peptide substrate [3879 multi-frame micrographs composed of 50 frames each in MRC format]
Publication:

Structural basis of substrate recognition by a polypeptide processing and secretion transporter

Kieuvongngam V, Olinares PDB, Palillo A, Oldham ML, Chait BT, Chen J

eLife 9 (2020)

PMID: 31934861

Related EMDB entry:
Deposited:
2021-09-10
Released:
2021-11-26
Last modified:
2021-11-26
Imageset size:
1.82 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned and uncorrected multiframe movies of PCAT1 bound to its CtA peptide substrate
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
3879
Image size:
(7420, 7676)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(0.545, 0.545)
Details:
The micrographs are the raw 50-frame tif movies collected with a super-resolution pixel size 0.545 Angstrom (1.09 Angstrom after binning). The dataset is separated into two subdirectories. The mrc movies has been compressed to bz2 format. The gain reference files are in a separate directory as the movie datasets
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