EMPIAR-10815 - Cryo electron tomograms of mouse DRG axons (dataset 1) [multiple data sets in MRC format]

EMPIAR-10815

Cryo electron tomograms of mouse DRG axons (dataset 1) [multiple data sets in MRC format]

Publication:

A cryo-ET survey of microtubules and intracellular compartments in mammalian axons

Foster HE, Ventura Santos C, Carter AP

Journal of Cell Biology 221 (2021)

PMID: 34878519

DOI: 10.1083/jcb.202103154

Related EMDB entry:

EMD-13601 EMD-13602 EMD-13600

Deposited:

2021-07-28

Released:

2021-12-17

Last modified:

2021-12-17

Imageset size:

200.79 GB

Imageset DOI:

10.6019/EMPIAR-10815

Experimental metadata:

Download xml json

Contains:

reconstructed volumes
tilt series

Image Sets

1. Tomograms of mouse dorsal root ganglion axons (dataset 1) binned by 4 and deconvolved

Category:

reconstructed volumes

Image format:

MRC

No. of images or tilt series:

Image size:

(927, 927)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(13.76, 13.76)

Details:

Reconstructed bin4 tomograms. Volumes were filtered using the Wiener-like deconvolution filter implemented in WARP (Tegunov 2018, https://github.com/dtegunov/tom\_deconv). Tomograms were reconstructed in IMOD by weighted back projection.

Files:

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2. Corrected, aligned, dose-filtered and order-sorted tilt series for mouse dorsal root ganglion axons (dataset 1)

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3710, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(3.44, 3.44)

Details:

Corrected, aligned, dose-filtered and sorted tilt series. The tilt series were generated from the raw frame images by correction for gain, defects and motion. In addition, tilt images were dose-filtered. Gain-correction and defects files are provided in the Raw_frames folder. Motion-correction was performed with alignframes (IMOD) operated in subTOM.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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