EMPIAR-10815
Cryo electron tomograms of mouse DRG axons (dataset 1) [multiple data sets in MRC format]
Publication:

A cryo-ET survey of microtubules and intracellular compartments in mammalian axons

Foster HE, Ventura Santos C, Carter AP

Journal of Cell Biology 221 (2021)

PMID: 34878519

Related EMDB entry:
Deposited:
2021-07-28
Released:
2021-12-17
Last modified:
2021-12-17
Imageset size:
200.79 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • reconstructed volumes
  • tilt series
1. Tomograms of mouse dorsal root ganglion axons (dataset 1) binned by 4 and deconvolved
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
30
Image size:
(927, 927)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(13.76, 13.76)
Details:
Reconstructed bin4 tomograms. Volumes were filtered using the Wiener-like deconvolution filter implemented in WARP (Tegunov 2018, https://github.com/dtegunov/tom\_deconv). Tomograms were reconstructed in IMOD by weighted back projection.
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2. Corrected, aligned, dose-filtered and order-sorted tilt series for mouse dorsal root ganglion axons (dataset 1)
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
30
Image size:
(3710, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(3.44, 3.44)
Details:
Corrected, aligned, dose-filtered and sorted tilt series. The tilt series were generated from the raw frame images by correction for gain, defects and motion. In addition, tilt images were dose-filtered. Gain-correction and defects files are provided in the Raw_frames folder. Motion-correction was performed with alignframes (IMOD) operated in subTOM.
Files:
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