EMPIAR-10604 - Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state [1768 multi-frame micrographs composed of 30 frames each in MRCS format]

EMPIAR-10604

Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state [1768 multi-frame micrographs composed of 30 frames each in MRCS format]

Publication:

Structural basis for a complex I mutation that blocks pathological ROS production

Yin Z, Burger N, Kula-Alwar D, Aksentijević D, Bridges HR, Prag H, Grba D, Viscomi C, James AJ, Mottahedin A, Kreig T, Murphy MP, Hirst J

Nature communications 12 (2021)

PMID: 33514727

DOI: 10.1038/s41467-021-20942-w

Related EMDB entry:

EMD-11810 EMD-4356

Deposited:

2020-12-07

Released:

2021-02-12

Last modified:

2021-02-12

Imageset size:

2.75 TB

Imageset DOI:

10.6019/EMPIAR-10604

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Single particle cryo-EM dataset of Mus musculus mitochondrial complex I in the deactive state

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

1768

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

Micrographs were imaged using a Gatan K2 summit camera in counting mode and GIF Quantum energy filter (20 eV slit width) on an FEI 300 keV Titan Krios microscope with a 100 μm objective aperture. 15 s exposures captured in 30 frames and a total dose of 50 electrons Å-2

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Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

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