EMPIAR-10597 - Human DPP9-CARD8 complex [multiple data sets in TIFF format]

EMPIAR-10597

Human DPP9-CARD8 complex [multiple data sets in TIFF format]

Publication:

Dipeptidyl peptidase 9 sets a threshold for CARD8 inflammasome formation by sequestering its active C-terminal fragment

Sharif H, Hollingsworth LR, Griswold AR, Hsiao JC, Wang Q, Bachovchin DA, Wu H

Immunity 54 (2021) 1392-1404.e10

PMID: 34019797

DOI: 10.1016/j.immuni.2021.04.024

Related EMDB entry:

EMD-22367

Deposited:

2020-12-15

Released:

2021-05-25

Last modified:

2022-02-15

Imageset size:

1.59 TB

Imageset DOI:

10.6019/EMPIAR-10597

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi frame micographs of CARD8-DPP9-noTILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3306

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.825, 0.825)

Details:

For CARD8-DPP9, 3,306 movies were collected at varying defocus levels ranging between −0.8 to −2.2 μm. We used image shift to record two shots for each of the four holes per stage movement. Movies were exposed with a total dose of 58.5 e-/Å2 for 2.22 s fractionated over 49 frames.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Unaligned multi frame micographs of CARD8-DPP9-apo-TILT

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

2488

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.825, 0.825)

Details:

For CARD8-DPP9, 2,488 movies at a stage tilt of 37° were collected at varying defocus levels ranging between
−1.5 to −3.0 μm. We used image shift to record two shots for each of the four holes per stage movement. Movies were exposed with a total dose of 64.99 e-/Å2 for 2.25 s fractionated over 49 frames for 37° stage tilt movies.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

Protein Data Bank Japan News

[2024-11-13]

236 new PDB entries have been released on 2024-11-13.

[2024-11-01]

A new portal integrating genome variants and protein structures has released

[2024-10-30]

[wwPDB] Deprecation of FTP File Download Protocol on November 1, 2024

[2024-10-25]

PDBj luncheon seminar at APBJC 2024

[2024-10-17]

[wwPDB] The Nobel Prize in Chemistry 2024