EMPIAR-10560 - Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana [multiple data sets in MRC format]

EMPIAR-10560

Nudaurelia capensis omega virus capsid: virus-like particles expressed in Nicotiana benthamiana [multiple data sets in MRC format]

Publication:

Plant-expressed virus-like particles reveal the intricate maturation process of a eukaryotic virus

Castells-Graells R, Ribeiro JRS, Domitrovic T, Hesketh EL, Scarff CA, Johnson JE, Ranson NA, Lawson DM, Lomonossoff GP

Communications biology 4 (2021)

PMID: 34031522

DOI: 10.1038/s42003-021-02134-w

Related EMDB entry:

EMD-11830

Deposited:

2020-11-17

Released:

2022-02-04

Last modified:

2022-02-04

Imageset size:

3.39 TB

Imageset DOI:

10.6019/EMPIAR-10560

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

MRC movies (Images-Disc1/*/FoilHole*Fractions.mrc) in this entry were compressed by bzip2 (pbzip2)
by EMPIAR Japan to save space and bandwidth.

Some programs (e.g. RELION >= 4.0.1 and CryoSPARC) can process compressed movies without explicit
decompression. For other programs, movies have to be decompressed beforehand. In this case,
you might want to convert decompressed MRC movies into TIFF to save your local storage.

Original size:   5,390,076,648 kilobytes
Compressed size: 3,432,762,789  kilobytes

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. unaligned movies

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2788

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.097, 1.097)

Details:

Micrographs are unaligned movies comprised of 59 frames each

data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.

data/motion_corrected_data/star_files - Star files from motion correction job.

Files:

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2. aligned and dose-weighted micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

2788

Image size:

(4096, 4096)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.0651, 1.0651)

Details:

Micrographs are averages of aligned and dose-weighted movies comprised of 59 frames

data/motion_corrected_data/shift_eps_files - EPS files showing motion correction trajectories for each movie in the dataset.

data/motion_corrected_data/star_files - Star files from motion correction job.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

See all citations

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