EMPIAR-10544 - Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases [multiple data sets in MRCS and MRC formats]

EMPIAR-10544

Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases [multiple data sets in MRCS and MRC formats]

Publication:

Cryo-EM structure of a dimeric B-Raf:14-3-3 complex reveals asymmetry in the active sites of B-Raf kinases

Kondo Y, Ognjenović J, Banerjee S, Karandur D, Merk A, Kulhanek K, Wong K, Roose JP, Subramaniam S, Kuriyan J

Science (New York, N.Y.) 366 (2019) 109-115

PMID: 31604311

DOI: 10.1126/science.aay0543

Related EMDB entry:

EMD-20708

Deposited:

2020-10-15

Released:

2022-06-22

Last modified:

2022-06-22

Imageset size:

934.30 GB

Imageset DOI:

10.6019/EMPIAR-10544

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

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Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of B-Raf:14-3-3 complex

Category:

micrographs - multiframe

Image format:

MRCS

No. of images or tilt series:

1539

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.532, 0.532)

Details:

The micrographs are the unaligned, unnormalized super-resolution movie frames (40 frames per micrograph). Total accumulated dose is 50 electrons/Å^2 over a 12 seconds exposure. GainRef.mrc can be used with 270 degrees rotation.

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2. Unaligned multi-frame micrographs of B-Raf:14-3-3 complex

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

1451

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.532, 0.532)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)