CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 1 [multiple data sets in TIFF, MRC and MRCS formats]
Publication:
Structures of α-synuclein filaments from multiple system atrophy
Movies were recorded automatically using EPU on a K3 camera in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 2.3 second movies contain 59 frames and the super resolution pixel size is 0.415 Å/px. The total dose per movie is ~49.2 e/Å^2. The gain reference image is included.
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Movie frames were corrected for beam-induced motion and dose-weighted using RELION’s motion-correction implementation with following arguments: --first_frame_sum 1 --last_frame_sum -1 --use_own --j 4 --bin_factor 2 --bfactor 150 --angpix 0.415 --voltage 300 --dose_per_frame 0.81 --preexposure 0 --patch_x 4 --patch_y 4 --gainref Gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting --save_noDW --only_do_unfinished --early_binning
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Bayesian polishing was performed in RELION-3.0. STAR file with the metadata of the final refined segments is included.
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Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.