EMPIAR-10357 - CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 1 [multiple data sets in TIFF, MRC and MRCS formats]

EMPIAR-10357

CryoEM dataset of sarkosyl-insoluble fractions from the putamen of multiple system atrophy brain of case 1 [multiple data sets in TIFF, MRC and MRCS formats]

Publication:

Structures of α-synuclein filaments from multiple system atrophy

Schweighauser M, Shi Y, Tarutani A, Kametani F, Murzin AG, Ghetti B, Matsubara T, Tomita T, Ando T, Hasegawa K, Murayama S, Yoshida M, Hasegawa M, Scheres SHW, Goedert M

Nature 585 (2020) 464-469

PMID: 32461689

DOI: 10.1038/s41586-020-2317-6

Related EMDB entry:

EMD-10650

Deposited:

2020-02-03

Released:

2020-08-17

Last modified:

2020-12-09

Imageset size:

3.19 TB

Imageset DOI:

10.6019/EMPIAR-10357

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame
picked particles - single frame - processed

Image Sets

1. Unaligned multi-frame movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

6936

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.415, 0.415)

Details:

Movies were recorded automatically using EPU on a K3 camera in super-resolution mode on a Titan Krios microscope operated at 300 kV (Cs=2.7) equipped with an energy filter using a slit width of 20 eV. The 2.3 second movies contain 59 frames and the super resolution pixel size is 0.415 Å/px. The total dose per movie is ~49.2 e/Å^2. The gain reference image is included.

Files:

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2. Dose-weighted aligned micrographs

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

6936

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.829, 0.829)

Details:

Movie frames were corrected for beam-induced motion and dose-weighted using RELION’s motion-correction implementation with following arguments:
--first_frame_sum 1 --last_frame_sum -1 --use_own --j 4 --bin_factor 2 --bfactor 150 --angpix 0.415 --voltage 300 --dose_per_frame 0.81 --preexposure 0 --patch_x 4 --patch_y 4 --gainref Gain.mrc --gain_rot 0 --gain_flip 0 --dose_weighting --save_noDW --only_do_unfinished --early_binning

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Polished particle stacks

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

3031

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.829, 0.829)

Details:

Bayesian polishing was performed in RELION-3.0. STAR file with the metadata of the final refined segments is included.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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