EMPIAR-10333
Single Particle Cryo-EM Reconstructions of Human FACT in Complex with Partially Assembled Sub-nucleosomes [multiple data sets in TIFF, MRC and MRCS formats]
Publication:

FACT caught in the act of manipulating the nucleosome

Liu YL, Zhou KZ, Zhang NZ, Wei HW, Tan YZT, Zhang ZZ, Carragher BC, Potter CSP, D'Arcy SD, Luger KL

Nature (2019)

PMID: 31775157

Related EMDB entry:
Deposited:
2019-10-21
Released:
2019-12-20
Last modified:
2021-11-08
Imageset size:
2.21 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
  • micrographs - single frame
  • picked particles - single frame - processed
1. Unaligned multi-frame micrographs
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
8319
Image size:
(3838, 3710)
Pixel type:
UNSIGNED 16 BIT INTEGER
Pixel spacing:
(1.0961, 1.0961)
Details:
Unaligned movies saved in TIF format. The first data collection (19feb16b) was performed using a dose of ~66.37 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~8.0 e–/pix/sec, using a set defocus range of -1.9 μm to -2.0 μm. The second data collection (19may31e) was performed using a dose of ~ 63.61 e-/Å2 across 50 frames (200 msec per frame) at a dose rate of ~7.6 e–/pix/sec, using a set defocus range of -1.5 μm to -2.5 μm. The gain reference images for each data collection are in the respective references folder.
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2. Aligned and dose-weighted micrographs
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
6934
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.0961, 1.0961)
Details:
Aligned and dose-weighted micrographs. Motion correction was performed using Relion 3.
Files:
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3. Final Particle Stacks for Class 1 and 2 with Euler Angles and Shifts
Category:
picked particles - single frame - processed
Image format:
MRCS
No. of images or tilt series:
2
Image size:
(200, 200)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.403008, 1.403008)
Details:
Final particle stacks for both class 1 and 2 maps with corresponding Relion .star file containing the refined Euler angles and shifts.
Files:
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Files:
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