EMPIAR-10270 - Single particle reconstruction of 52 kDa biotin-bound state streptavidin at 3.2 Angstrom resolution [3309 multi-frame micrographs composed of 32 frames each in MRC format]

EMPIAR-10270

Single particle reconstruction of 52 kDa biotin-bound state streptavidin at 3.2 Angstrom resolution [3309 multi-frame micrographs composed of 32 frames each in MRC format]

Publication:

Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution

Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J, Wang HW

Nature communications 10 (2019)

PMID: 31160591

DOI: 10.1038/s41467-019-10368-w

Related EMDB entry:

EMD-0689

Deposited:

2019-03-08

Released:

2019-06-07

Last modified:

2019-06-07

Imageset size:

6.03 TB

Imageset DOI:

10.6019/EMPIAR-10270

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Uncorrected biotinbound state streptavidin movie stacks, binning 2 from super-resolution stacks.

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3309

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.5265, 0.5265)

Details:

biotin_SA_run_data.star
45686 particles for final 3D reconstruction.

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Citations

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

Williams HM, Curtis WA, Haubner M, Hruby J, Drabbels M, Lorenz UJ. (2025)

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