EMPIAR-10265 - LAT1-CD98hc bound to HBJ127 Fab and MEM-108 Fab [1869 multi-frame micrographs composed of 40 frames each in TIFF format]

EMPIAR-10265

LAT1-CD98hc bound to HBJ127 Fab and MEM-108 Fab [1869 multi-frame micrographs composed of 40 frames each in TIFF format]

Publication:

Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc

Lee Y, Kusakizako T, Nureki O

Nature structural & molecular biology (2019)

PMID: 31160781

DOI: 10.1038/s41594-019-0237-7

Related EMDB entry:

EMD-9850

Deposited:

2019-03-24

Released:

2019-06-21

Last modified:

2019-06-21

Imageset size:

1.04 TB

Imageset DOI:

10.6019/EMPIAR-10265

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

1869

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.7625, 0.7625)

Details:

The pixel size (0.7625 A/px) is for super-resolution pixels. The defect file (defect.txt) and the gain reference (gain.mrc) are also uploaded. In UCSF MotionCor2, you have to apply "-RotGain 1 -FlipGain 2". The final set of particles and orientations are stored in particle.star. The total exposure is 50.2 electrons per square angstrom in a 15.6 second exposure.

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Citations

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PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

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Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

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