EMPIAR-10265
LAT1-CD98hc bound to HBJ127 Fab and MEM-108 Fab [1869 multi-frame micrographs composed of 40 frames each in TIFF format]
Publication:

Cryo-EM structure of the human L-type amino acid transporter 1 in complex with glycoprotein CD98hc

Lee Y, Kusakizako T, Nureki O

Nature structural & molecular biology (2019)

PMID: 31160781

Related EMDB entry:
Deposited:
2019-03-24
Released:
2019-06-21
Last modified:
2019-06-21
Imageset size:
969.88 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
1869
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.7625, 0.7625)
Details:
The pixel size (0.7625 A/px) is for super-resolution pixels. The defect file (defect.txt) and the gain reference (gain.mrc) are also uploaded. In UCSF MotionCor2, you have to apply "-RotGain 1 -FlipGain 2". The final set of particles and orientations are stored in particle.star. The total exposure is 50.2 electrons per square angstrom in a 15.6 second exposure.
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