EMPIAR-10203
Structure of the Macrobrachium rosenbergii Nodavirus: A new genus within the Nodaviridae? [2459 micrographs in MRC format]
Publication:

Structure of the Macrobrachium rosenbergii nodavirus: A new genus within the Nodaviridae?

Ho KL, Gabrielsen M, Beh PL, Kueh CL, Thong QX, Streetley J, Tan WS, Bhella D

PLoS biology 16 (2018)

PMID: 30346944

Related EMDB entry:
Deposited:
2018-08-02
Released:
2018-10-25
Last modified:
2022-02-08
Imageset size:
130.44 GB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - single frame
1. Motion corrected micrographs of Macrobrachium rosenbergii nodavirus virus-like particles
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
2459
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.06, 1.06)
Details:
To collect high-resolution data on MrNV VLPs, grids were imaged at the electron bioimaging centre (eBIC), Diamond Light Source (UK) using an FEI Titan Krios operated at 47,170 X magnification. Images were recorded on a Gatan K2 BioQuantum energy-filtered direct detector camera operated in zero-loss imaging mode with a slit width of 20 eV. Five second exposures were recorded in electron counting mode, at a frame-rate of four frames per second and a dose rate of 1.8 electrons/pixel/frame. The pixel size was 1.06 Å/pixel.
Motion correction was performed in Motioncor2.
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