EMPIAR-10185 - T. acidophilum 20S proteasome core movies obtained using Talos Arctica operating at 200 kV equipped with a K2 – stage position used for exposure target navigation [317 multi-frame micrographs composed of 68 frames each in MRC format]

EMPIAR-10185

T. acidophilum 20S proteasome core movies obtained using Talos Arctica operating at 200 kV equipped with a K2 – stage position used for exposure target navigation [317 multi-frame micrographs composed of 68 frames each in MRC format]

Publication:

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

Herzik Jr MA, Wu M, Lander GC

Nature methods 14 (2017) 1075-1078

PMID: 28991891

DOI: 10.1038/nmeth.4461

Related EMDB entry:

EMD-8741

Deposited:

2018-05-03

Released:

2018-05-11

Last modified:

2018-05-11

Imageset size:

140.91 GB

Imageset DOI:

10.6019/EMPIAR-10185

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (*frames.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   1,191,402,923 kilobytes
Compressed size:   147,307,946 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Raw, unaligned movie stacks of T. acidophilum 20S proteasome core acquired on a Talos Arctica using a K2 direct electron detector - stage position used for exposure navigation

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

317

Image size:

(7420, 7676)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.46, 0.46)

Details:

Raw, unaligned super-resolution frame stacks (68 frames) of T. acidophilum 20S proteasome core in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector - stage position used for exposure navigation. Images used for normalizations are also included.

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Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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