EMPIAR-10185
T. acidophilum 20S proteasome core movies obtained using Talos Arctica operating at 200 kV equipped with a K2 – stage position used for exposure target navigation [317 multi-frame micrographs composed of 68 frames each in MRC format]
Publication:

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

Herzik Jr MA, Wu M, Lander GC

Nature methods 14 (2017) 1075-1078

PMID: 28991891

Related EMDB entry:
Deposited:
2018-05-03
Released:
2018-05-11
Last modified:
2018-05-11
Imageset size:
140.91 GB
Imageset DOI:
Experimental metadata:
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Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies (*frames.mrc) in this entry were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:   1,191,402,923 kilobytes
Compressed size:   147,307,946 kilobytes
Contains:
  • micrographs - multiframe
1. Raw, unaligned movie stacks of T. acidophilum 20S proteasome core acquired on a Talos Arctica using a K2 direct electron detector - stage position used for exposure navigation
Category:
micrographs - multiframe
Image format:
MRC
No. of images or tilt series:
317
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.46, 0.46)
Details:
Raw, unaligned super-resolution frame stacks (68 frames) of T. acidophilum 20S proteasome core in vitreous ice imaged on a Talos Arctica using a K2 direct electron detector - stage position used for exposure navigation. Images used for normalizations are also included.
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