EMPIAR-10180 - Structure of a pre-catalytic spliceosome [stack of 327490 particles in MRCS format]

EMPIAR-10180

Structure of a pre-catalytic spliceosome [stack of 327490 particles in MRCS format]

Publication:

Structure of a pre-catalytic spliceosome

Plaschka C, Lin PC, Nagai K

Nature 546 (2017) 617-621

PMID: 28530653

DOI: 10.1038/nature22799

Related EMDB entry:

EMD-3686 EMD-3687 EMD-3685 EMD-3688 EMD-3684 EMD-3682 EMD-3683

Deposited:

2018-03-27

Released:

2018-05-30

Last modified:

2018-08-30

Imageset size:

135.81 GB

Imageset DOI:

10.6019/EMPIAR-10180

Experimental metadata:

Download xml json

Contains:

picked particles - single frame - processed

Image Sets

1. Polished parcitles

Category:

picked particles - single frame - processed

Image format:

MRCS

No. of images or tilt series:

327490

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.699, 1.699)

Details:

Shiny particles after particle polishing used for the Nature paper were further down-sampled to 1.699 Å/px and windowed to 320 px. These particles were used in the Multibody paper.

The particle STAR file after consensus refinement, masks and initial references for four bodies and a body STAR file are also included. Readers can reproduce the multibody refinement in the paper using these inputs.

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Citations

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

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In situ structure determination of conformationally flexible targets using nextPYP.

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EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

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