EMPIAR-10168 - RNA Polymerase III pre-initiation complex [multiple data sets in TIFF and MRC formats]

EMPIAR-10168

RNA Polymerase III pre-initiation complex [multiple data sets in TIFF and MRC formats]

Publication:

Molecular mechanism of promoter opening by RNA polymerase III

Vorländer MK, Khatter H, Wetzel R, Hagen WJH, Müller CW

Nature 553 (2018) 295-300

PMID: 29345638

DOI: 10.1038/nature25440

Related EMDB entry:

EMD-4184 EMD-4180 EMD-4183 EMD-4182

Deposited:

2018-03-23

Released:

2018-06-18

Last modified:

2018-06-18

Imageset size:

3.99 TB

Imageset DOI:

10.6019/EMPIAR-10168

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Movie frames

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3612

Image size:

(7676, 7420)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(0.675, 0.675)

Details:

TIFs were recorded in super resolution mode on the K2. File "SuperRef_96-1_000_Apr26_17.48.03.mrc", which is the GainRef file of the K2 detector converted to MRC format, is required for unpacking the TIF files with MotionCor2.

Files:

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2. Aligned sums of the movie frames without doseweighting

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3612

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Doseweighted aligned sums of the movie frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3612

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Movie frames

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4783

Image size:

(7676, 7420)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(0.675, 0.675)

Details:

Second set of images for entry EMPIAR-10168. TIFs were recorded in super resolution mode on the K2 with a frame dose of 2.0 electrons per frame. File "SuperRef_112-1_000_Apr03_18.03.03.mrc", which is the GainRef file of the K2 detector converted to MRC format, is required for unpacking the TIF files with MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Aligned sums of the movie frames without doseweighting

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4783

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

Second set of images for entry EMPIAR-10168. The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. Doseweighted aligned sums of the movie frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4783

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

Second set of images for entry EMPIAR-10168. The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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