EMPIAR-10168 - RNA Polymerase III pre-initiation complex [multiple data sets in TIFF and MRC formats]

EMPIAR-10168

RNA Polymerase III pre-initiation complex [multiple data sets in TIFF and MRC formats]

Publication:

Molecular mechanism of promoter opening by RNA polymerase III

Vorländer MK, Khatter H, Wetzel R, Hagen WJH, Müller CW

Nature 553 (2018) 295-300

PMID: 29345638

DOI: 10.1038/nature25440

Related EMDB entry:

EMD-4184 EMD-4180 EMD-4183 EMD-4182

Deposited:

2018-03-23

Released:

2018-06-18

Last modified:

2018-06-18

Imageset size:

3.99 TB

Imageset DOI:

10.6019/EMPIAR-10168

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Movie frames

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3612

Image size:

(7676, 7420)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(0.675, 0.675)

Details:

TIFs were recorded in super resolution mode on the K2. File "SuperRef_96-1_000_Apr26_17.48.03.mrc", which is the GainRef file of the K2 detector converted to MRC format, is required for unpacking the TIF files with MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Aligned sums of the movie frames without doseweighting

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3612

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Doseweighted aligned sums of the movie frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

3612

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Movie frames

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

4783

Image size:

(7676, 7420)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(0.675, 0.675)

Details:

Second set of images for entry EMPIAR-10168. TIFs were recorded in super resolution mode on the K2 with a frame dose of 2.0 electrons per frame. File "SuperRef_112-1_000_Apr03_18.03.03.mrc", which is the GainRef file of the K2 detector converted to MRC format, is required for unpacking the TIF files with MotionCor2.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

5. Aligned sums of the movie frames without doseweighting

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

4783

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

Second set of images for entry EMPIAR-10168. The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

6. Doseweighted aligned sums of the movie frames

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

4783

Image size:

(3838, 3710)

Pixel type:

UNSIGNED 32 BIT INTEGER

Pixel spacing:

(1.35, 1.35)

Details:

Second set of images for entry EMPIAR-10168. The files were obtained with MotionCor2

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Quick Links

Talks and Tutorials

EMPIAR Quick tour

Statistics

Publications

Re-use case study

EMPIAR in the news

Claim entries to your ORCiD

COVID-19 Data Portal

EMDB

PDBe

BioImage Archive

EMNavigator@PDBj

PDBj

RCSB@PDBj

EMDataResoruce@RCSB,EMDB,NCMI

Citations

Structural basis for HIV-1 antagonism of host APOBEC3G via Cullin E3 ligase.

Ito F, Alvarez-Cabrera AL, Liu S, Yang H, Shiriaeva A, Zhou ZH, Chen XS. (2023)

The 2023 Nucleic Acids Research Database Issue and the online molecular biology database collection.

Rigden DJ, Fernández XM. (2023)

EMPIAR: the Electron Microscopy Public Image Archive.

Iudin A, Korir PK, Somasundharam S, Weyand S, Cattavitello C, Fonseca N, Salih O, Kleywegt GJ, Patwardhan A. (2023)

CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM.

Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

Structure of a RecT/Redβ family recombinase in complex with a duplex intermediate of DNA annealing.

Caldwell BJ, Norris AS, Karbowski CF, Wiegand AM, Wysocki VH, Bell CE. (2022)

See all citations

Protein Data Bank Japan News

[2024-04-24]

354 new PDB entries have been released on 2024-04-24.

[2024-04-09]

[wwPDB] CASP16 Call for Targets

[2024-04-02]

Announcement of the PDBj Newsletter Vol.24 release

[2024-03-21]

[wwPDB] Paper Published on NMR Restraint Validation

[2024-03-13]

[wwPDB] Paper Published on CryoEM Archiving and Validation Recommendations

[2024-03-06]

[wwPDB] Poster Prize Awarded at The Biophysical Society Meeting

[2024-02-15]

Notice of Service Suspension (ASH, DASH, SeSAW, MAFFTash, Spanner, SFAS)

[2024-02-13]

[wwPDB] Biocurator Milestone: >10,000 Depositions Processed

[2024-02-06]

[wwPDB] Preprint Published on NMR Restraint Validation

[2024-02-05]

[wwPDB] Preprint Published on CryoEM Archiving and Validation Recommendations

See all news