EMPIAR-10141 - CryoET of apoferritin with 0.5 mM TCEP single particle [multiple data sets in MRC format]

EMPIAR-10141

CryoET of apoferritin with 0.5 mM TCEP single particle [multiple data sets in MRC format]

Publication:

Routine single particle CryoEM sample and grid characterization by tomography

Noble AJ, Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ, Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET, Rice WJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B

eLife 7 (2018)

PMID: 29809143

DOI: 10.7554/elife.34257

Related EMDB entry:

EMD-7150

Deposited:

2017-12-06

Released:

2018-04-05

Last modified:

2022-07-21

Imageset size:

35.29 GB

Imageset DOI:

10.6019/EMPIAR-10141

Experimental metadata:

Download xml json

Version history:

2018-04-05

REL

The depositor added tomography particle picks.

Contains:

tilt series
picked particles - single frame - processed

Image Sets

1. Tilt-series image stacks

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 4984)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.34, 2.34)

Details:

Not frame-aligned.
.mdoc files included.

Files:

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Download a list of selected files.

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rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Appion-Protomo tilt-series alignments

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.34, 2.34)

Details:

Includes a full tilt-series alignment run for each tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Manual particle picking in Dynamo

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

Binned by 4 manual particle picking of the tomogram using Dynamo in TBL and OMD formats that relate to the corresponding .mrc file. Manual picking was performed to accurately distinguish between particles adsorbed to the air-water interfaces, non-adsorbed particles, and particles over or adsorbed to carbon.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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