EMPIAR-10141 - CryoET of apoferritin with 0.5 mM TCEP single particle [multiple data sets in MRC format]

EMPIAR-10141

CryoET of apoferritin with 0.5 mM TCEP single particle [multiple data sets in MRC format]

Publication:

Routine single particle CryoEM sample and grid characterization by tomography

Noble AJ, Dandey VP, Wei H, Brasch J, Chase J, Acharya P, Tan YZ, Zhang Z, Kim LY, Scapin G, Rapp M, Eng ET, Rice WJ, Cheng A, Negro CJ, Shapiro L, Kwong PD, Jeruzalmi D, des Georges A, Potter CS, Carragher B

eLife 7 (2018)

PMID: 29809143

DOI: 10.7554/elife.34257

Related EMDB entry:

EMD-7150

Deposited:

2017-12-06

Released:

2018-04-05

Last modified:

2022-07-21

Imageset size:

35.29 GB

Imageset DOI:

10.6019/EMPIAR-10141

Experimental metadata:

Download xml json

Version history:

2018-04-05

REL

The depositor added tomography particle picks.

Contains:

tilt series
picked particles - single frame - processed

Image Sets

1. Tilt-series image stacks

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 4984)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(2.34, 2.34)

Details:

Not frame-aligned.
.mdoc files included.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

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Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

2. Appion-Protomo tilt-series alignments

Category:

tilt series

Image format:

MRC

No. of images or tilt series:

Image size:

(3712, 3838)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(2.34, 2.34)

Details:

Includes a full tilt-series alignment run for each tilt-series. To view properly, navigate to the protomo_alignments folder in Appion-Protomo.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Manual particle picking in Dynamo

Category:

picked particles - single frame - processed

Image format:

MRC

No. of images or tilt series:

Image size:

(None, None)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(None, None)

Details:

Binned by 4 manual particle picking of the tomogram using Dynamo in TBL and OMD formats that relate to the corresponding .mrc file. Manual picking was performed to accurately distinguish between particles adsorbed to the air-water interfaces, non-adsorbed particles, and particles over or adsorbed to carbon.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Serra Lleti JM, Steyer AM, Schieber NL, Neumann B, Tischer C, Hilsenstein V, Holtstrom M, Unrau D, Kirmse R, Lucocq JM, Pepperkok R, Schwab Y. (2023)

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