EMPIAR-10099 - Cryo-EM structure of Hrd1 and Hrd3 complex [multiple data sets in MRC format]

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EMPIAR-10099

Cryo-EM structure of Hrd1 and Hrd3 complex [multiple data sets in MRC format]

Publication:

Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3

Schoebel S, Mi W, Stein A, Ovchinnikov S, Pavlovicz R, DiMaio F, Baker D, Chambers MG, Su H, Li D, Rapoport TA, Liao M

Nature (2017)

PMID: 28682307

DOI: 10.1038/nature23314

Related EMDB entry:

EMD-8639 EMD-8638

Deposited:

2017-05-17

Released:

2017-08-29

Last modified:

2017-08-29

Imageset size:

708.27 GB

Imageset DOI:

10.6019/EMPIAR-10099

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
picked particles - multiframe - processed

Image Sets

1. summed frame micrographs of Hrd1/Hrd3 complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5361

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

Files are named from Ucsf_stack_0001.mrc to Ucsf_stack_5361.mrc
These are raw micrographs after drift correction from MotionCor2 without dose weighting.

Files:

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2. dose-weighted summed frame micrographs of Hrd1/Hrd3 complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

5361

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

Files are named from Ucsf_stack_0001_DW.mrc to Ucsf_stack_5361_DW.mrc
These are summed micrographs after drift correction from MotionCor2 with dose-weighting

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. picked particles of Hrd1/Hrd3 complex

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

1015288

Image size:

(192, 192)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.35, 1.35)

Details:

particles are in three files
Hrd13_July25-26_fixs5_i_n1.mrc
Hrd13_July27-28_fixs5_i_n1.mrc
Hrd13_July29-31_fixs5_i_n1.mrc
total particle number from above three files is 1015288.
corresponding star files are available as well

Under folder Hrd13_svco-files_bin3_1015288 are coordinate files of picked particles, and files are SPIDER format. The coordinates are for images binned by 3, so if the users want to box particles from raw images, they need to multiple 3 for all coordinates to get the right value.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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