EMPIAR-10099
Cryo-EM structure of Hrd1 and Hrd3 complex [multiple data sets in MRC format]
Publication:

Cryo-EM structure of the protein-conducting ERAD channel Hrd1 in complex with Hrd3

Schoebel S, Mi W, Stein A, Ovchinnikov S, Pavlovicz R, DiMaio F, Baker D, Chambers MG, Su H, Li D, Rapoport TA, Liao M

Nature (2017)

PMID: 28682307

Related EMDB entry:
Deposited:
2017-05-17
Released:
2017-08-29
Last modified:
2017-08-29
Imageset size:
708.27 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - multiframe - processed
1. summed frame micrographs of Hrd1/Hrd3 complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
5361
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.35, 1.35)
Details:
Files are named from Ucsf_stack_0001.mrc to Ucsf_stack_5361.mrc
These are raw micrographs after drift correction from MotionCor2 without dose weighting.
Files:
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2. dose-weighted summed frame micrographs of Hrd1/Hrd3 complex
Category:
micrographs - single frame
Image format:
MRC
No. of images or tilt series:
5361
Image size:
(3838, 3710)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.35, 1.35)
Details:
Files are named from Ucsf_stack_0001_DW.mrc to Ucsf_stack_5361_DW.mrc
These are summed micrographs after drift correction from MotionCor2 with dose-weighting
Files:
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3. picked particles of Hrd1/Hrd3 complex
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
1015288
Image size:
(192, 192)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.35, 1.35)
Details:
particles are in three files
Hrd13_July25-26_fixs5_i_n1.mrc
Hrd13_July27-28_fixs5_i_n1.mrc
Hrd13_July29-31_fixs5_i_n1.mrc
total particle number from above three files is 1015288.
corresponding star files are available as well

Under folder Hrd13_svco-files_bin3_1015288 are coordinate files of picked particles, and files are SPIDER format. The coordinates are for images binned by 3, so if the users want to box particles from raw images, they need to multiple 3 for all coordinates to get the right value.
Files:
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Files:
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