EMPIAR-10076
CryoEM Dataset of L17-Depleted 50S Ribosomal Intermediates [stack of 131899 particles in MRC format]
Publication:

Modular Assembly of the Bacterial Large Ribosomal Subunit

Davis JH, Tan Y, Carragher B, Potter CS, Lyumkis D, Williamson JR

Cell 167 (2016) 1610-1622.e15

Deposited:
2016-10-31
Released:
2016-12-02
Last modified:
2016-12-02
Imageset size:
50.34 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • picked particles - multiframe - unprocessed
1. Particle stack of L17-depleted 50S Ribosomal Assembly Intermediates
Category:
picked particles - multiframe - unprocessed
Image format:
MRC
No. of images or tilt series:
131899
Image size:
(320, 320)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.31, 1.31)
Details:
131899 particle stack has been obtained from 2479 images with 50 frames per image, using frames from 1 to 50, aligned on a per-particle basis using Rubinstein's particle polishing software, exposure-weighted, not inverted, normalized. Stack contains a heterogeneous mixture of particles. In order to account for highly preferred orientation of the specimen, data was acquired using tilts ranging from 10-60 degrees, in addition to untilted images at 0 degrees. Parameter file for Frealign and Relion (stack is not yet normalized to Relion format) provided.
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