EMPIAR-10076 - CryoEM Dataset of L17-Depleted 50S Ribosomal Intermediates [stack of 131899 particles in MRC format]

EMPIAR-10076

CryoEM Dataset of L17-Depleted 50S Ribosomal Intermediates [stack of 131899 particles in MRC format]

Publication:

Modular Assembly of the Bacterial Large Ribosomal Subunit

Davis JH, Tan Y, Carragher B, Potter CS, Lyumkis D, Williamson JR

Cell 167 (2016) 1610-1622.e15

DOI: 10.1016/j.cell.2016.11.020

Related EMDB entry:

EMD-8446 EMD-8443 EMD-8445 EMD-8444 EMD-8441 EMD-8434 EMD-8440 EMD-8442 EMD-8448 EMD-8449 EMD-8447 EMD-8452 EMD-8453 EMD-8455 EMD-8456 EMD-8451 EMD-8450 EMD-8457

Deposited:

2016-10-31

Released:

2016-12-02

Last modified:

2016-12-02

Imageset size:

50.34 GB

Imageset DOI:

10.6019/EMPIAR-10076

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - unprocessed

Image Sets

1. Particle stack of L17-depleted 50S Ribosomal Assembly Intermediates

Category:

picked particles - multiframe - unprocessed

Image format:

MRC

No. of images or tilt series:

131899

Image size:

(320, 320)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.31, 1.31)

Details:

131899 particle stack has been obtained from 2479 images with 50 frames per image, using frames from 1 to 50, aligned on a per-particle basis using Rubinstein's particle polishing software, exposure-weighted, not inverted, normalized. Stack contains a heterogeneous mixture of particles. In order to account for highly preferred orientation of the specimen, data was acquired using tilts ranging from 10-60 degrees, in addition to untilted images at 0 degrees. Parameter file for Frealign and Relion (stack is not yet normalized to Relion format) provided.

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Citations

Small LEA proteins mitigate air-water interface damage to fragile cryo-EM samples during plunge freezing.

Abe KM, Li G, He Q, Grant T, Lim CJ. (2024)

Outer membrane protein assembly mediated by BAM-SurA complexes.

Fenn KL, Horne JE, Crossley JA, Böhringer N, Horne RJ, Schäberle TF, Calabrese AN, Radford SE, Ranson NA. (2024)

Ubiquitinated histone H2B as gatekeeper of the nucleosome acidic patch.

Hicks CW, Rahman S, Gloor SL, Fields JK, Husby NL, Vaidya A, Maier KE, Morgan M, Keogh MC, Wolberger C. (2024)

Molecular recognition of an odorant by the murine trace amine-associated receptor TAAR7f.

Gusach A, Lee Y, Khoshgrudi AN, Mukhaleva E, Ma N, Koers EJ, Chen Q, Edwards PC, Huang F, Kim J, Mancia F, Veprintsev DB, Vaidehi N, Weyand SN, Tate CG. (2024)

The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.

Kofler L, Grundmann L, Gerhalter M, Prattes M, Merl-Pham J, Zisser G, Grishkovskaya I, Hodirnau VV, Vareka M, Breinbauer R, Hauck SM, Haselbach D, Bergler H. (2024)

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