EMPIAR-10072
Structural basis for dynamic regulation of the human 26S proteasome [multiple data sets in SPIDER and MRC formats]
Publication:

Structural basis for dynamic regulation of the human 26S proteasome

Chen S, Wu J

Proceedings of the National Academy of Sciences of the United States of America 113 (2016) 12991-12996

PMID: 27791164

Deposited:
2016-08-17
Released:
2016-11-10
Last modified:
2016-11-10
Imageset size:
2.01 TB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • micrographs - single frame
  • picked particles - multiframe - processed
1. Drift-corrected micrographs of human 26S proteasome holoenzyme
Category:
micrographs - single frame
Image format:
SPIDER
No. of images or tilt series:
6967
Image size:
(7420, 7676)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(0.86, 0.86)
Details:
The micrograph are the average from 30 re-aligned movie frames to minimize any effect of drift or sample movement, accumulating 30 electron per squared Angstrom in a 6 second exposure.
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2. Classified particle datasets for the human proteasome in four conformational states
Category:
picked particles - multiframe - processed
Image format:
MRC
No. of images or tilt series:
506186
Image size:
(None, None)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(None, None)
Details:
The description of particle stack: (1) 26S.mrcs 237,083 particles for double cap with dimension of 640*640. (2) 26S_A.mrcs 85,420 particles for double cap at SA state, the dimension of particles is 640*640; (3) 26SH_A.mrcs 139,236 particles for RP-CP complex at SA state, the dimension of particles is 360*360; (4) 26SH_B.mrcs 18,443 particles for RP-CP complex at SB state, the dimension of particles is 360*360; (5) 26SH_C.mrcs 10,622 particles for RP-CP complex at SC state, the dimension of particles is 360*360; (6) 26SH_D.mrcs 14,382 particles for RP-CP complex at SD state, the dimension of particles is 360*360; the pixel size of all the particles stack is 0.86 angstrom.
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