EMPIAR-10049 - Cryo-EM Structures of Synaptic RAG1-RAG2 Complex [multiple data sets in MRC format]

EMPIAR-10049

Cryo-EM Structures of Synaptic RAG1-RAG2 Complex [multiple data sets in MRC format]

Publication:

Molecular Mechanism of V(D)J Recombination from Synaptic RAG1-RAG2 Complex Structures.

Ru H, Chambers MG, Fu TM, Tong AB, Liao M, Wu H

Cell 163 (2015) 1138-1152

PMID: 26548953

DOI: doi:10.1016/j.cell.2015.10.055

Related EMDB entry:

EMD-6487

Deposited:

2015-11-19

Released:

2015-11-24

Last modified:

2015-11-24

Imageset size:

70.81 GB

Imageset DOI:

10.6019/EMPIAR-10049

Experimental metadata:

Download xml json

Contains:

picked particles - multiframe - processed
micrographs - single frame

Image Sets

1. RAG SEC particle stack

Category:

picked particles - multiframe - processed

Image format:

MRC

No. of images or tilt series:

108544

Image size:

(192, 192)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.23, 1.23)

Details:

"allimg.star" is the Relion star file containing the EM and defocus parameters for each particle.
"ragSEC_150309.mrcs" is the particle stack from the 1st data set after summing all 30 frames, and "ragSEC_150311_f03-16.mrcs" is the particle stack from the 1st data set after summing frames 3-16.
"ragSEC_150311.mrcs" and "ragSEC_150311_f03-16.mrcs" are the particle stacks from the 2nd data set.
"ragSEC_volume_half1.mrc" and "ragSEC_volume_half2.mrc" are the two final unfiltered half maps.

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2. Summed micrographs from drift-corrected multi-frame microsgraphs of RAG SEC (1st data set)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

330

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.23, 1.23)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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3. Summed micrographs from drift-corrected multi-frame microsgraphs of RAG SEC (2nd data set)

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

350

Image size:

(3838, 3710)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.23, 1.23)

Details:

---

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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Citations

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Kim K, Li H, Clarke OB. (2025)

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CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Laser flash melting cryo-EM samples to overcome preferred orientation.

Straub MS, Harder OF, Mowry NJ, Barrass SV, Hruby J, Drabbels M, Lorenz UJ. (2025)

In situ structure determination of conformationally flexible targets using nextPYP.

Liu HF, Zhou Y, Huang Q, Martin J, Bartesaghi A. (2025)

EMICSS: added-value annotations for EMDB entries.

Duraisamy AK, Fonseca N, Kleywegt GJ, Patwardhan A, Morris KL. (2025)

Global conformations of <i>Pichia pastoris</i> complex I are distinguished by the binding of a unique interdomain bridging subunit.

Lee CS, Grba DN, Wright JJ, Ivanov BS, Hirst J. (2025)

Overcoming Preferred Orientation in Cryo-EM With Ultrasonic Excitation During Vitrification

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