EMPIAR-10048 - Cryo-electron tomogram of Chlamydia trachomatis with type III secretion system in contact with HeLa cell [1 class averages in MRC format]

EMPIAR-10048

Cryo-electron tomogram of Chlamydia trachomatis with type III secretion system in contact with HeLa cell [1 class averages in MRC format]

Publication:

Structure of a bacterial type III secretion system in contact with a host membrane in situ

Nans A, Kudryashev M, Saibil HR, Hayward RD

Nat Commun 6 (2015) 10114-10114

PMID: 26656452

DOI: doi:10.1038/ncomms10114

Related EMDB entry:

EMD-3217

Deposited:

2016-01-27

Released:

2016-01-27

Last modified:

2016-01-27

Imageset size:

14.57 GB

Imageset DOI:

10.6019/EMPIAR-10048

Experimental metadata:

Download xml json

Contains:

class averages

Image Sets

1. Aligned multi-frame micrographs that comprise a tilt series of Chlamydia trachomatis type III secretion systems in contact with a HeLa cell

Category:

class averages

Image format:

MRC

No. of images or tilt series:

Image size:

(3708, 3838)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(5.4, 5.4)

Details:

Tilt images were collected from -60 to +45 in 3 degree increments. Total dose was 54 electrons/A2. Nominal defocus was 10 microns and CTF correction was applied in IMOD.

The reconstructed data is in HeLa_host9a.rec and consists of 952 unsigned byte 3708x3838 images.

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