micrographs - multiframe

EER

10943

(4096, 4096)

UNSIGNED BYTE

(0.5248, 0.5248)

Raw, unaligned EER movies (not gain-corrected) recorded with EPU on a Talos Arctica (Thermo Fisher Scientific) operated at 200 kV (FEG, Cs 2.7 mm) and equipped with a Falcon 4i detector. Nominal magnification 190,000x; pixel size 0.5248 A/px (0.525 A/px in EMDB) for the 4096 x 4096 images, i.e. without EER upsampling. Each movie contains 666 EER frames; total fluence approximately 40 e-/A^2 (38.6-40.1 e-/A^2 according to the EER headers). Nominal defocus range -0.8 to -2.4 um. Folder layout: data/movies/batch_01-03 contains the 10,943 EER movies; data/epu_metadata/batch_01-03 contains the 10,942 corresponding EPU XML metadata files (one movie has no XML file); data/gain_reference/20240318_163908_EER_GainReference.gain is the gain reference to be applied to the EER movies. These movies were processed in cryoSPARC 4.6 to obtain EMD-52764, EMD-52765, EMD-52766 and EMD-52840 (PDB 9IF4). Acquisition values were read from the raw data headers and EPU metadata; Table 1 of the associated publication lists 120,000x magnification, 46 e-/A^2 and a defocus range of -0.8 to -2.2 um.

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