EMPIAR-13971 - Cryo-EM of Mtb RNAP elongation complex (substrate loading mimic) [34503 multi-frame micrographs composed of 35 frames each in TIFF format]

EMPIAR-13971

Cryo-EM of Mtb RNAP elongation complex (substrate loading mimic) [34503 multi-frame micrographs composed of 35 frames each in TIFF format]

Publication:

RNA polymerase inhibitors reveal active-site motions essential for the nucleotide addition cycle

Dhingra Y., Landick R., Campbell E.A., Darst S.A.

Proceedings of the National Academy of Sciences 123 (2026) e2609228123-e2609228123

PMID: 42378287

DOI: 10.1073/pnas.2609228123

Related EMDB entry:

EMD-75288 EMD-75286 EMD-75287

Deposited:

2026-09-03

Released:

2026-09-24

Last modified:

2026-09-24

Imageset size:

5.92 TB

Imageset DOI:

10.6019/EMPIAR-13971

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned raw movies

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

34503

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.847, 0.847)

Details:

Grids were imaged using a 300 kV Titan Krios (ThermoFisher Scientific) equipped with
a K3 camera (Gatan). Images were recorded using SerialEM with a pixel size of 0.847 Å/px over
a nominal defocus range of −0.6 to −2.0 μm and 20 eV energy filter slit width. A total of 34,503
(AAP-SO2 minus) gain-normalized movies were recorded in counting
mode (image dimensions of 4092 × 5760 px) with 27.5 e−/px/s (at the camera) in dose fractionation
mode of 0.04 s over a 1.4 s exposure (35 frames) to give a total dose of 50.7 e−/Å2.

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CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

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