EMPIAR-13566
In situ cryo-ET dataset of primary human T cells [multiple data sets in MRC format]
Publication:

Molecular architecture of heterochromatin at the nuclear periphery of primary human cells

Kreysing JP, Cruz-Leon S, Betz J, Penzo C, Majtner T, Schreiber M, Turonova B, Lusic M, Hummer G, Beck M

bioRxiv (2025)

Related EMDB entry:
Deposited:
2026-05-14
Released:
2026-06-25
Last modified:
2026-06-25
Imageset size:
331.97 GB
Imageset DOI:
Experimental metadata:
Download xml json
Contains:
  • tilt series
  • reconstructed volumes
1. Raw, unaligned, gain-corrected frames of primary human T cells
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
14
Image size:
(4096, 4096)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(1.895, 1.895)
Details:
The mdoc metadata files are also provided.
Files:
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2. Tilt cleaned, dose-filtered tilt-series of primary human T cells
Category:
tilt series
Image format:
MRC
No. of images or tilt series:
14
Image size:
(4096, 4096)
Pixel type:
32 BIT FLOAT
Pixel spacing:
(1.895, 1.895)
Details:
These tilt stacks were used for tomogram reconstruction in IMOD and novaCTF. The relevant alignment files (.xf and .tlt) are provided in the alignment_files folder.
Files:
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3. Reconstructed tomograms (4x binning) of primary human T cells
Category:
reconstructed volumes
Image format:
MRC
No. of images or tilt series:
14
Image size:
(1024, 1024)
Pixel type:
SIGNED 16 BIT INTEGER
Pixel spacing:
(7.58, 7.58)
Details:
All tomograms were reconstructed in IMOD with 4x binning and a sirt-like filter. Tomogram 019 was additionally denoised with cryoCARE and IsoNet2.
Files:
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