EMPIAR-13496 - Cryo-EM of SARS-CoV-2 frame shift stimulation element [multiple data sets in MRC format]

EMPIAR-13496

Cryo-EM of SARS-CoV-2 frame shift stimulation element [multiple data sets in MRC format]

Publication:

Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome

Zhang K, Zheludev IN, Hagey RJ, Haslecker R, Hou YJ, Kretsch R, Pintilie GD, Rangan R, Kladwang W, Li S, Wu MT, Pham EA, Bernardin-Souibgui C, Baric RS, Sheahan TP, D'Souza V, Glenn JS, Chiu W, Das R

Nature structural & molecular biology 28 (2021) 747-754

PMID: 34426697

DOI: 10.1038/s41594-021-00653-y

Related EMDB entry:

EMD-22296 EMD-22297

Deposited:

2026-01-06

Released:

2026-04-28

Last modified:

2026-04-28

Imageset size:

3.69 TB

Imageset DOI:

10.6019/EMPIAR-13496

Experimental metadata:

Download xml json

Compressed by PDBj:

Image formats and pixel types shown below are those of the original, non-compressed files.

Movies in this entry (data/*/*.mrc) were compressed to TIFF by EMPIAR Japan to save space and bandwidth.
The compression is lossless in that all pixel intensities remain exactly the same after decompression.
However, some metadata in the MRC header were lost.
The original files before compression are available from EMPIAR EBI.

Original size:  14,739,882,580 kilobytes
Compressed siz:  3,958,988,992 kilobytes

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

10010

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

The final datasets in this study were imaged in a Titan Krios cryo-electron microscope (Thermo Fisher Scientific) operated at 300 kV with a GIF energy filter (Gatan) at a magnification of ×165,000 (corresponding to a calibrated sampling of 0.82 Å per pixel) for both samples. Micrographs were recorded by EPU software (Thermo Fisher Scientific) with a Gatan K2 Summit direct electron detector, where each image was composed of 30 individual frames with an exposure time of 6 s and an exposure rate of 8.3 electrons per second per Å2.

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2. Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

13155

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

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3. Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element Tagged with ATP-TTR3 nanostructure

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

2714

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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4. Unaligned multiframe micrographs of SARS-CoV-2 Frameshift Stimulation Element Tagged with ATP-TTR3 nanostructure

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

9455

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.82, 0.82)

Details:

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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