Cryo-EM structure of the type I and IVb pilus from enterotoxigenic Escherichia coli [6900 multi-frame micrographs composed of 50 frames each in TIFF format]
Publication:
High-resolution cryo-EM analysis visualizes hydrated type I and IV pilus structures from enterotoxigenic Escherichia coli
Kawahara K, Oki H, Iimori M, Muramoto R, Imai T, Gerle C, Shigematsu H, Matsuda S, Iida T, Nakamura S
Structure (London, England : 1993)33 (2025) 1040-1050000.0
- Microscope: CRYO ARM™ 300 electron microscope (JEOL) - Detector: K3 direct electron detector (Gatan) - Acceleration voltage (kV): 300 - The pixel size: 0.752 Å/pix. - Spherical aberration (Cs) : 2.7 - Nominal Defocus (μm): 1.4 - 1.6 - Average electron dose per image (e-/A2): 53.77 - Number of frames per image: 50 - Please use "CountCDSRef_2022-07-08_18.16.20_CFAIII_150-1_0000.mrc" as a gain reference without rotation or flipping in cryoSPARC.
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