Cryo-EM micrographs of the clathrin adaptor complex AP-2 and the assembly chaperone CCDC32; dataset contains multiple assembly and conformational states [multiple data sets in TIFF format]
Publication:
CCDC32 collaborates with the membrane to assemble the AP-2 clathrin adaptor complex
This deposition contains two datasets, collected on the same microscope with the same settings. The datasets come from two grids made during the same freezing session, differing only in the amount of time the proteins were allowed to incubate before freezing (dataset 1 = short incubation; dataset 2 = long incubation).
Aligned, dose-weighted micrographs from cryoSPARC v4.7.0 are also included for each dataset.
The dataset contains at least three distinct assemblies: AP-2 in the closed conformation; AP-2 bound to CCDC32 in a "bowl" conformation; and AP-2 bound to CCDC32 in the "cargo-like" conformation. The datasets only differ in the relative proportion of these three assemblies.
keV - 300 Cs - 2.7 Ac - 0.1 Angpix = 0.657 Frames - 60 Total dose - 60 e-/A2
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This deposition contains two datasets, collected on the same microscope with the same settings. The datasets come from two grids made during the same freezing session, differing only in the amount of time the proteins were allowed to incubate before freezing (dataset 1 = short incubation; dataset 2 = long incubation).
Aligned, dose-weighted micrographs from cryoSPARC v4.7.0 are also included for each dataset.
The dataset contains at least three distinct assemblies: AP-2 in the closed conformation; AP-2 bound to CCDC32 in a "bowl" conformation; and AP-2 bound to CCDC32 in the "cargo-like" conformation. The datasets only differ in the relative proportion of these three assemblies.
keV - 300 Cs - 2.7 Ac - 0.1 Angpix = 0.657 Frames - 60 Total dose - 60 e-/A2
Files:
Available download options:
This entry cannot be downloaded from PDBj.
Archives and downloads the selected files into an uncompressed zip file.
Depending on the number and size of files to be downloaded, this can take an enormous amount of time.
Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.
We recommend using rsync, aspera, globus, etc.
Download a list of selected files.
It is possible to download files by specifying the file list with rsync command, etc.
