EMPIAR-13235
Unaligned Movies of E. coli grown at 25°C clarified cell lysate [7908 multi-frame micrographs composed of 45 frames each in TIFF format]
Publication:

Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states

May MB, Lopez Perez GS, Davis JH

Proceedings of the National Academy of Sciences (PNAS) (---)

PMID: 40894545

Related EMDB entry:
Deposited:
2026-01-01
Released:
2026-02-11
Last modified:
2026-02-11
Imageset size:
1.96 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Name: Unaligned Movies of E. coli grown at 25°C clarified cell lysate
Category:
micrographs - multiframe
Image format:
TIFF
No. of images or tilt series:
7908
Image size:
(5760, 4092)
Pixel type:
UNSIGNED BYTE
Pixel spacing:
(1.06, 1.06)
Details:
Nominal Defocus range (min, max, step): -0.03,-0.2 to -2 in steps of 0.2
Total exposure on specimen: 45.1838 el/A2
Exposure time: 3.15 s
Nominal pixel size: 1.06 A
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3900 V
Microscope / Detector: Krios G3i / K3
Files:
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