EMPIAR-13232 - Unaligned Movies of E. coli grown at 37°C clarified cell lysate [9748 multi-frame micrographs composed of 45 frames each in TIFF format]

EMPIAR-13232

Unaligned Movies of E. coli grown at 37°C clarified cell lysate [9748 multi-frame micrographs composed of 45 frames each in TIFF format]

Publication:

Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states

May MB, Gabriella GS, Davis JH

Proceedings of the National Academy of Sciences (PNAS) (---)

PMID: 40894545

DOI: 10.1101/2025.08.21.669550

Related EMDB entry:

Deposited:

2025-12-31

Released:

2026-02-13

Last modified:

2026-02-13

Imageset size:

2.38 TB

Imageset DOI:

10.6019/EMPIAR-13232

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned Movies of E. coli grown at 37°C clarified cell lysate

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

9748

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(1.06, 1.06)

Details:

Nominal Defocus range (min, max, step): -0.03,-0.2 to -2 in steps of 0.2
Total exposure on specimen: 45.1838 el/A2
Exposure time: 3.15 s
Nominal pixel size: 1.06 A
Objective lens spherical aberration (Cs): 2.7 mm
Acceleration voltage: 300 kV
Extraction voltage: 3900 V
Microscope / Detector: Krios G3i / K3

Files:

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Citations

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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