EMPIAR-13196 - CryoEM structure of cyclised H-pilus mutant (D69R) [600 multi-frame micrographs composed of 50 frames each in MRC format]

EMPIAR-13196

CryoEM structure of cyclised H-pilus mutant (D69R) [600 multi-frame micrographs composed of 50 frames each in MRC format]

Publication:

R27 pilus biogenesis is promiscuous and electrostatic perturbations impair functionality

Ishimoto N, Beis K

(---)

Related EMDB entry:

EMD-65236

Deposited:

2025-08-28

Released:

2026-01-26

Last modified:

2026-01-26

Imageset size:

937.56 GB

Imageset DOI:

10.6019/EMPIAR-13196

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of H-pilus mutant (D69R)

Category:

micrographs - multiframe

Image format:

MRC

No. of images or tilt series:

600

Image size:

(4096, 4096)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(1.192, None)

Details:

---

Files:

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

Poudel B, Gyawali R, Dhakal A, Cheng J, Xu D. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

CryoWriter: a robotic solution for improved Cryo-EM grid preparation.

Kv C, Ekundayo B, Di Fabrizio M, Mohammed I, Radecke J, Stahlberg H, Kube M. (2026)