EMPIAR-13172 - Cryogenic transmission electron microscopy images of extracellular vesicles isolated from conditioned media of pancreatic cancer and normal pancreatic epithelial cell lines [3 micrographs in TIFF format]

EMPIAR-13172

Cryogenic transmission electron microscopy images of extracellular vesicles isolated from conditioned media of pancreatic cancer and normal pancreatic epithelial cell lines [3 micrographs in TIFF format]

Publication:

Extracellular vesicle derived miRNA-182-5p educates macrophages towards an immunosuppressive phenotype in pancreatic cancer

Singh BS, Gaur PG, Bose PB, Zhang YJ, Li YL, Zhang ZZ, Kandhavelu JK, Klotzbier WK, Jayatilake MJ, Bansal SB, Farhan MF, Deol SD, Banerjee PPB, Unger KU, Gupta SG, Verma VV, Cheema AKC

Signal Transduction and Targeted Therapy (---)

Related EMDB entry:

Deposited:

2025-12-13

Released:

2025-12-24

Last modified:

2025-12-24

Imageset size:

96.07 MB

Imageset DOI:

10.6019/EMPIAR-13172

Experimental metadata:

Download xml json

Contains:

micrographs - single frame

Image Sets

1. Extracellular vesicles (EVs) isolated from PANC-1, PPCL-68, and hTERT-HPNE cell lines conditioned media

Category:

micrographs - single frame

Image format:

TIFF

No. of images or tilt series:

Image size:

(4096, 4096)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(3.82, 3.82)

Details:

EV samples (∼3.5 μL) were loaded onto glow-discharged, perforated carbon-coated grids (2/1-3C C-Flat; Protochips, Raleigh, NC), blotted with filter paper, and rapidly plunged into liquid ethane. These grids were stored in liquid nitrogen. Before imaging, the grids were placed on cryo-specimen holder (626 cryo-specimen holder, Gatan, Warrendale, PA) and maintained at -180°C. The digital micrographs were captured using a Tecnai F20 Twin transmission electron microscope (ThermoFisher Scientific, Hillsboro, OR) equipped with a Gatan US4000 CCD or a Teitz XF416 camera.

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Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

DiameTR: A cryo-EM tool for diameter sorting of tubular samples.

Peng R, Elkhaligy H, Grant T, Stagg SM. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

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