EMPIAR-13163 - PglZ in complex with BrxB-BrxC fusion from the Acinetobacter BREX system [multiple data sets in MRC and TIFF formats]

EMPIAR-13163

PglZ in complex with BrxB-BrxC fusion from the Acinetobacter BREX system [multiple data sets in MRC and TIFF formats]

Publication:

Competing forms of protein-protein association and DNA binding exhibited by BrxC from the BREX phage restriction system

Doyle LD

(---)

Related EMDB entry:

EMD-74400

Deposited:

2025-12-11

Released:

2026-04-16

Last modified:

2026-04-16

Imageset size:

4.23 TB

Imageset DOI:

10.6019/EMPIAR-13163

Experimental metadata:

Download xml json

Contains:

micrographs - single frame
micrographs - multiframe

Image Sets

1. Motion Corrected micrographs for the BrxC-BrxB/PglZ complex

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

3964

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(1.122, 1.122)

Details:

Micrographs are averages motion corrected by WARP, binning pixels from 0.561 to 1.122 Å/px. Voltage 200 kV, Cs 2.7, Total exposure dose 50. Prior to motion correction, the gain was corrected.

Files:

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2. Unaligned multi-frame micrographs of the BrxC-BrxB/PglZ complex

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

3964

Image size:

(11520, 8184)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.561, 0.561)

Details:

The data is raw, multi-frame movies without any correction applied. Data was collected in SerialEM as LZW compressed TIFFs.

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

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Citations

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ICECREAM: high-fidelity equivariant cryo-electron tomography.

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CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)