EMPIAR-13154 - Single-particle cryo-EM data of the Hsp60 chaperonin complex [multiple data sets in TIFF and MRC formats]

EMPIAR-13154

Single-particle cryo-EM data of the Hsp60 chaperonin complex [multiple data sets in TIFF and MRC formats]

Publication:

Structural remodeling of the mitochondrial protein biogenesis machinery under proteostatic stress

Ehses K, López-Alonso JP, Antico O, Azem A, Muquit MMK, Ubarretxena-Belandia I, Fernández-Busnadiego R, Lang Y, Rudack T

Science Advances (---)

Related EMDB entry:

EMD-54901 EMD-54900 EMD-54902 EMD-54898 EMD-54899 EMD-54903

Deposited:

2025-11-26

Released:

2026-01-25

Last modified:

2026-01-25

Imageset size:

43.62 TB

Imageset DOI:

10.6019/EMPIAR-13154

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
picked particles - single frame - unprocessed

Image Sets

1. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (1 minute incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

11796

Image size:

(5760, 4092)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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2. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (7 minutes incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12055

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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3. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (7 minutes incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

8012

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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4. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (30 minutes incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

28004

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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5. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (30 seconds incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

5710

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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6. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (1 minute incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

12751

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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7. Unaligned multi-frame micrographs of Hsp60 + Hsp10 + MgATP (30 seconds incubation time)

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

21773

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

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8. particles for ATP single ring, ADP single ring and ATP half football

Category:

picked particles - single frame - unprocessed

Image format:

MRC

No. of images or tilt series:

95147

Image size:

(400, 400)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

Files:

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9. particles for ATP football, ATP double ring and ATP bullet

Category:

picked particles - single frame - unprocessed

Image format:

MRC

No. of images or tilt series:

95321

Image size:

(600, 600)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.8238, 0.8238)

Details:

---

Files:

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Citations

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Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)

Ultrathin liquid cells for microsecond time-resolved cryo-EM.

Curtis WA, Wenz J, Krüger CR, Barrass SV, Drabbels M, Lorenz UJ. (2026)

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

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Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

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