EMPIAR-13134 - Electron cryomicroscopy of NSF, the minimal binary SNARE complex of SNAP-25 and syntaxin-1a, and alpha-SNAP under non-hydrolyzing conditions [multiple data sets in TIFF and MRC formats]

EMPIAR-13134

Electron cryomicroscopy of NSF, the minimal binary SNARE complex of SNAP-25 and syntaxin-1a, and alpha-SNAP under non-hydrolyzing conditions [multiple data sets in TIFF and MRC formats]

Publication:

Structural remodeling of target-SNARE protein complexes by NSF enables synaptic transmission

White KI, Khan YA, Qiu K, Balaji A, Couoh-Cardel S, Esquivies L, Pfuetzner RA, Diao J, Brunger AT

Nature communications 16 (2025)

PMID: 40993127

DOI: 10.1038/s41467-025-62764-0

Related EMDB entry:

EMD-71601 EMD-71600

Deposited:

2025-09-04

Released:

2026-01-07

Last modified:

2026-01-07

Imageset size:

8.61 TB

Imageset DOI:

10.6019/EMPIAR-13134

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe
micrographs - single frame

Image Sets

1. Unaligned multi-frame micrographs of NSF, α-SNAP, and the minimal binary SNARE complex of SNAP-25 and syntaxin-1a under non-hydrolyzing conditions

Category:

micrographs - multiframe

Image format:

TIFF

No. of images or tilt series:

14257

Image size:

(5760, 4096)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.548, 0.548)

Details:

---

Files:

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2. Gain reference

Category:

micrographs - single frame

Image format:

MRC

No. of images or tilt series:

Image size:

(5760, 4092)

Pixel type:

32 BIT FLOAT

Pixel spacing:

(0.548, 0.548)

Details:

---

Files:

This entry cannot be downloaded from PDBj.

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

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Citations

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

PERC: a suite of software tools for the curation of cryoEM data with application to simulation, modeling and machine learning.

Costa-Gomes B, Greer J, Juraschko N, Parkhurst J, Mirecka J, Famili M, Rangel-Smith C, Strickson O, Lowe A, Basham M, Burnley T. (2025)

DiameTR: A cryo-EM tool for diameter sorting of tubular samples.

Peng R, Elkhaligy H, Grant T, Stagg SM. (2025)

Self-supervised learning for generalizable particle picking in cryo-EM micrographs.

Zamanos A, Koromilas P, Bouritsas G, Kastritis PL, Panagakis Y. (2025)

Localization of macromolecules in crowded cellular cryo-electron tomograms from extremely sparse labels.

Uddin MR, Ahmed AY, Tabib HMS, Tahmid MT, Alam MZU, Freyberg Z, Xu M. (2025)

Structural insight into bicarbonate-mediated carboxylation by human vitamin K-dependent carboxylase.

Wu K, Wang Z, Yao D, Li S, Wang X, Zhang Y, Cao M, Shen Y, Xing S, Wu J, Lei M, Lan P. (2025)

Conserved hydrophilic checkpoints tune FocA-mediated formate:H<sup>+</sup> symport.

Tüting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL. (2025)

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