EMPIAR-13085 - GLIC F116A mutant in MSP1E3D1 nanodisc at pH 2.5 [11131 multi-frame micrographs composed of 1575 frames each in EER format]

EMPIAR-13085

GLIC F116A mutant in MSP1E3D1 nanodisc at pH 2.5 [11131 multi-frame micrographs composed of 1575 frames each in EER format]

Publication:

Asymmetric gating of a homopentameric ion channel GLIC revealed by cryo-EM

Li Z, Bharambe N, Manishrao Lande K, Feddersen B, Manikkoth Balakrishna A, Biggin PC, Sahu G, Basak S

Proceedings of the National Academy of Sciences of the United States of America 122 (2025)

PMID: 41129221

DOI: 10.1073/pnas.2512811122

Related EMDB entry:

EMD-62941

Deposited:

2025-01-27

Released:

2025-12-03

Last modified:

2025-12-03

Imageset size:

7.30 TB

Imageset DOI:

10.6019/EMPIAR-13085

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. GLIC F116A mutant in MSP1E3D1 nanodisc at pH 2.5

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

11131

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.76, 0.76)

Details:

Raw movies collected by Titan Krios with Falcon4i camera at 165kx magnification, pixel size 0.76ang/pix, total dose 70e.

Files:

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Citations

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Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

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He L, Bartesaghi A. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)