EMPIAR-13084 - GLIC Y251A mutant in MSP1E3D1 nanodisc at pH 2.5 [10188 multi-frame micrographs composed of 1251 frames each in EER format]

EMPIAR-13084

GLIC Y251A mutant in MSP1E3D1 nanodisc at pH 2.5 [10188 multi-frame micrographs composed of 1251 frames each in EER format]

Publication:

Asymmetric gating of a homopentameric ion channel GLIC revealed by cryo-EM

Li Z, Bharambe N, Manishrao Lande K, Feddersen B, Manikkoth Balakrishna A, Biggin PC, Sahu G, Basak S

Proceedings of the National Academy of Sciences of the United States of America 122 (2025)

PMID: 41129221

DOI: 10.1073/pnas.2512811122

Related EMDB entry:

EMD-62942

Deposited:

2025-01-24

Released:

2025-12-03

Last modified:

2025-12-03

Imageset size:

6.24 TB

Imageset DOI:

10.6019/EMPIAR-13084

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. GLIC Y251A mutant in MSP1E3D1 nanodisc at pH 2.5

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

10188

Image size:

(None, None)

Pixel type:

UNSIGNED BYTE

Pixel spacing:

(0.76, 0.76)

Details:

Raw movies collected by Titan Krios with Falcon4i camera at 165kx magnification, pixel size 0.76, total dose 70e.

Files:

Archives and downloads the selected files into an uncompressed zip file.

Depending on the number and size of files to be downloaded, this can take an enormous amount of time. Also, this feature is unstable and may not work properly, and checksums of downloaded files are not verified.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

Browse All Files

Quick Links

Browse EMPIAR

Sample Preparation Widget

Volume Browser

Tomo Viewer Prototype

Claim entries to your ORCiD

Talks and Tutorials

EMPIAR Quick tour

Publications

Re-use study

Statistics

EMPIAR in the news

COVID-19 Data Portal

EMDB

BioImage Archive

PDBe

EMNavigator@PDBj

PDBj

Citations

CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

Deng Y, Wang S, Xiang M, Li Y, Zhuo L, Cao D, Fu X, Zou Q. (2026)

prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

He L, Bartesaghi A. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

Point cloud deformation modeling for particle selection following cryo-EM 2D classification.

Wang X, Mo Z, Li F, Zhang F, Wan X. (2026)

ICECREAM: high-fidelity equivariant cryo-electron tomography.

Kishore V, Debarnot V, Righetto RD, Engel BD, Dokmanić I. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)