EMPIAR-13076
cryo-EM data for Drosophila Insulin Receptor ectodomain with DILP2 hormone [multiple data sets in EER format]
Publication:

Towards understanding of one receptor:many hormones paradigm: cryo-EM structures of Drosophila Insulin Receptor ectodomain with DILP2 hormone

Shafi T, Moroz OV, Jenkins HT, Chechik M, Isupov MN, Brzozowski AM

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Related EMDB entry:
Deposited:
2025-10-23
Released:
2026-05-31
Last modified:
2026-05-31
Imageset size:
3.36 TB
Imageset DOI:
Experimental metadata:
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Contains:
  • micrographs - multiframe
1. Unaligned multi-frame micrographs of Drosophila Insulin Receptor ectodomain complex with DILP2 hormone
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
1068
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 32 BIT INTEGER
Pixel spacing:
(0.574, 0.574)
Details:
Title: cryo-EM data for Drosophila Insulin Receptor ectodomain with DILP2 hormone
Microscope/Detector: TFS Glacios / Falcon IV
Grid 1 contains 1068 of total
12103 movies, corresponding to the micrograms selected after CTFFIND
Calibrated pixel size: 0.574 A
Flux: 6.27 e-/pixel/s
Total fluence 50 e-/A^2
Data were collected from two separate grids corresponding to different stages of the project timeline described in the associated publication.
The two datasets are provided in separate subdirectories (Grid 1 and Grid 2) under the EMPIAR root
Data from both datasets were joined and used at the final stages of the project,resulting in two different structures: entry emd-52236, PDB 9HKT, 3.76A and enry EMD-52310, PDB 9HNI, 6.25A
three other files under EMPIAR root are the following
emd-52236_run_data.star contains metadata for 127081 particles from Refine3D for EMD-52236, pdb 9HKT
emd-52310_run_data.star contains metadata for 57317 particles from Refine3D for EMD-52310, pdb 9HNI
dmirdilp2_GainReference.gain
Files:
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2. Unaligned multi-frame micrographs of Drosophila Insulin Receptor ectodomain complex with DILP2 hormone
Category:
micrographs - multiframe
Image format:
EER
No. of images or tilt series:
11035
Image size:
(4096, 4096)
Pixel type:
UNSIGNED 32 BIT INTEGER
Pixel spacing:
(0.574, 0.574)
Details:
Same as described for Grid 1 (and README file), but contains the remaining 11035 micrographs
Files:
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