EMPIAR-13004 - Electron tomography of mitochondria from adult and aged mice hearts [multiple data sets in TIFF format]

EMPIAR-13004

Electron tomography of mitochondria from adult and aged mice hearts [multiple data sets in TIFF format]

Publication:

Multiscale mitochondrial cristae remodeling links Opa1 downregulation to reduced OXPHOS capacity in aged hearts

Molina-Riquelme I, Barrientos G, Breitsprecher L, Gómez W, Díaz-Castro F, Morris S, Almarza G, Del Campo A, Garrido-Olivares L, Verdejo HE, Psathaki OE, Busch KB, Eisner V

Proceedings of the National Academy of Sciences of the United States of America 123 (2025)

PMID: 41468425

DOI: 10.1073/pnas.2508911123

Related EMDB entry:

Deposited:

2025-09-24

Released:

2025-12-17

Last modified:

2026-01-05

Imageset size:

523.11 MB

Imageset DOI:

10.6019/EMPIAR-13004

Experimental metadata:

Download xml json

Contains:

tilt series
reconstructed volumes

Image Sets

1. Tilt-series of a cardiac mitochondrion from an adult mouse

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

129

Image size:

(1024, 1024)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(20.4761, 20.4761)

Details:

Tilt-series (±60°, 1° increment) were obtained in TALOS F200C G2 equipped with a Ceta 16M CMOS camera, with 28000X magnification and 200 kV. Stage drift was corrected in Inspect 3D software.

Files:

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2. Tilt-series of a cardiac mitochondrion from an aged mouse

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

241

Image size:

(512, 512)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(40.9521, 40.9521)

Details:

Tilt-series (±60°, 0.5° increment) were obtained in TALOS F200C G2 equipped with a Ceta 16M CMOS camera, with 28000X magnification and 200 kV. Stage drift was corrected in Inspect 3D software.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

3. Reconstructed volume of a cardiac mitochondrion from an adult mouse

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

Image size:

(1024, 1024)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(20.4761, 20.4761)

Details:

Volume was reconstructed using SIRT algorithm with 20 iterations in Inspect 3D software.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

4. Reconstructed volume of a cardiac mitochondrion from an aged mouse

Category:

reconstructed volumes

Image format:

TIFF

No. of images or tilt series:

Image size:

(512, 512)

Pixel type:

SIGNED 16 BIT INTEGER

Pixel spacing:

(40.9521, 40.9521)

Details:

Volume was reconstructed using SIRT algorithm with 20 iterations in Inspect 3D software.

Files:

Archives and downloads the selected files into an uncompressed zip file.

We recommend using rsync, aspera, globus, etc.

Download a list of selected files.

It is possible to download files by specifying the file list with rsync command, etc.

rsync -av --files-from=(path-to-filelist) rsync://empiar.pdbj.org/empiar/archive (path-to-destination)

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