EMPIAR-13000 - Human TRPM4 ion channel in MSP2N2 lipid nanodisc in a calcium-bound state [7945 multi-frame micrographs composed of 40 frames each in EER format]

EMPIAR-13000

Human TRPM4 ion channel in MSP2N2 lipid nanodisc in a calcium-bound state [7945 multi-frame micrographs composed of 40 frames each in EER format]

Publication:

MAASTY: A (dis)ordered copolymer for structural determination of human membrane proteins in native nanodiscs

Pugh CF, Feilen LP, Živković D, Præstegaard KF, Sideris C, Borthwick NJ, de Lichtenberg C, Bolla JR, Autzen AAA, Autzen HE

Nature Communications (---)

Related EMDB entry:

EMD-55036

Deposited:

2025-09-09

Released:

2026-03-01

Last modified:

2026-03-01

Imageset size:

3.78 TB

Imageset DOI:

10.6019/EMPIAR-13000

Experimental metadata:

Download xml json

Contains:

micrographs - multiframe

Image Sets

1. Unaligned multi-frame micrographs of hTRPM4 in MSP2n" nanodiscs in Ca2+-bound state

Category:

micrographs - multiframe

Image format:

EER

No. of images or tilt series:

7945

Image size:

(4096, 4096)

Pixel type:

BIT

Pixel spacing:

(0.725, 0.725)

Details:

These are the EER files from Falcon 4i detector - raw micrographs, not motion corrected, acquired with EPU and a total dose of 52 e-/Å2 (1.3 e-/Å2 pr frame)
Gain reference image can be found in the /data/ directory
job028_particles.star contains all picked particles
job034_partciles.star contains the subset of articles from 2D classification (first selection)
job055_particles.star contains the subset of particles from the best classes after 3D classification that was further refined to the final reconstruction (second selection)

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Citations

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Deep-learning methods for contrast enhancement and artifact reduction in cryo-electron tomography: a systematic analysis of the state of the art and proposed improvements.

Jones HN, Deshmukh A, Pande K. (2026)

CryoGS: High-Quality Cryo-EM Homogeneous Reconstruction by Gaussian Splatting

Zeng B, Liu S, Cheng S, Xu G, Fan H. (2026)

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection.

Schäfer JH, Calza A, Hom K, Damodar P, Peng R, Bogdanović N, Lander GC, Stagg SM, Cianfrocco MA. (2025)

Ultrathin liquid cells for microsecond time-resolved cryo-EM.

Curtis WA, Wenz J, Krüger CR, Barrass SV, Drabbels M, Lorenz UJ. (2026)

GTpick: A deep neural network for Cryo-EM particle detection.

Ni S, Yang C, Liu Y, Zhang Y, Shi Y, Qian A, Kong R, Chang S. (2025)

Species-specific structural adaptation of the potyviral coat protein in virions and virus-like particles.

Koritnik N, Kežar A, Kavčič L, Žnidarič MT, Leonardi A, De S, Pollari M, Mäkinen K, Podobnik M. (2026)

High-resolution ab initio reconstruction enables cryo-EM structure determination of small particles

Kim K, Li H, Clarke OB. (2025)

CLEM-Reg: an automated point cloud-based registration algorithm for volume correlative light and electron microscopy.

Krentzel D, Elphick M, Domart MC, Peddie CJ, Laine RF, Shand C, Henriques R, Collinson LM, Jones ML. (2025)

CryoEMNet driven symmetry-aware molecular reconstruction through deep learning enhanced electron microscopy.

Arora S, Pan SH, Kumar S, Singh SK, Chauhan U, Alhalabi W, Arya V, Alsulami BS, Hsu CH, Chui KT, Gupta BB. (2025)

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