EMPIAR-12908 - Cryo-tomography of 1.4 nm-HAN-488 labeled ribosomes inside cells (ExoSloNano) [49 tilt series in TIFF format]

EMPIAR-12908

Cryo-tomography of 1.4 nm-HAN-488 labeled ribosomes inside cells (ExoSloNano) [49 tilt series in TIFF format]

Publication:

Cryo-tomography of 1.4 nm-HAN-488 labeled ribosomes inside cells

Young LN, Sherard A, Bennett E, Rosen MK, Giraldez AJ, Villa E, Zhou H, Shaikh F, Hutchings J, Riggi M, Narasimhan M, Flaherty WA

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Related EMDB entry:

Deposited:

2025-06-09

Released:

2025-11-14

Last modified:

2025-11-14

Imageset size:

28.08 GB

Imageset DOI:

10.6019/EMPIAR-12908

Experimental metadata:

Download xml json

Contains:

tilt series

Image Sets

1. Cryo-FIB-ET on 1.4 nm HAN-488 delivered to HEK 293T L29-Halo cells - frames assoc. with tilt series

Category:

tilt series

Image format:

TIFF

No. of images or tilt series:

Image size:

(4096, 5760)

Pixel type:

UNSIGNED 16 BIT INTEGER

Pixel spacing:

(1.068, 1.068)

Details:

Tilt series from cryo-FIB-ET data from lamella from HEK 293 L29-HaloTag cells delivered with 1.4 nm HAN-488 nanogold label.

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Citations

AlignPCA-2D: PCA-reduced Euclidean vector alignment for 2D classification in cryo-EM.

Ramírez-Aportela E, Zarrabeitia OL, Fonseca YC, Ceska T, Subramaniam S, Carazo JM, Sorzano COS. (2026)

A variational framework with composite sparse regularization for cryo-electron tomography reconstruction.

Yu C, Xu Z, Zeng Q, Wan X, El-Messiry H, Zhang F, Han R. (2026)

Helical reconstruction of amyloids in cryoSPARC.

Schaefer JH, O'Neill RT, Donnelly JP, Powers ET, Kelly JW, Lander GC. (2026)

An unattended image-processing pipeline for on-the-fly quality assessment and 3D exploration in cryo-EM.

Marchán Torres D, Conesa P, Garcia A, Iceta M, Broche L, Gragera M, Linares R, Kwong HS, Chichón FJ, Svensson O, Arranz R, Effantin G, Kandiah E, Carazo JM, Sorzano COS. (2026)

CryoFSL: an annotation-efficient, few-shot learning framework for robust protein particle picking in cryo-electron microscopy micrographs.

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prismPYP: Power-spectrum and image domain learning for self-supervised micrograph evaluation.

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CryoSIP: unleashing protein high-resolution Cryo-EM via semantic-instance collaborative picking.

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Unbend, correction of local beam-induced sample motion in cryo-EM images using a 3D spline model.

Kong L, Zottig X, Elferich J, Grigorieff N. (2026)

CryoVirusDB: An Annotated Dataset for AI-Based Virus Particle Identification in Cryo-EM Micrographs.

Gyawali R, Dhakal A, Wang L, Cheng J. (2026)

Chart builder: an interactive tool for user driven data visualization in the electron microscopy data bank.

Fonseca N, Duraisamy AK, Wang Z, Somasundharam S, Tayebinia M, de Oliveira LC, Ma M, Turner J, Patwardhan A, Kleywegt GJ, Hartley M, Morris KL. (2026)

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